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Top 5 Amino Acid & Peptide Calming Supplements for Dogs in Canada (2025) — Veterinarian-Recommended, Clinically Informed Picks to Support Stress and Anxiety

Published on Thursday, August 21, 2025

Formulations centered on amino acids and bioactive peptides — such as L-tryptophan, L-theanine, GABA, and alpha-casozepine — are designed to support neurotransmitter balance and stress regulation in dogs. These products appeal to Canadian pet owners because they offer targeted, evidence-informed biochemical support as an alternative or complement to behavioral training and, where appropriate, prescription medications. Canadian consumers increasingly prefer veterinarian-recommended options with transparent ingredient sourcing, clear dosing, and scientifically referenced claims. Availability through veterinary clinics, specialty retailers, and online Canadian distributors, along with rising interest in natural-origin peptides and amino acids, has made this category a practical choice for owners seeking measurable support for situational anxiety, noise sensitivity, travel stress, and general nervousness.

Quick Menu

1. Best Alpha-Casozepine Calm

2. Best Vet-Formulated Tryptophan Blend

3. Best L-Theanine Chewable

4. Best Budget Calming Chew

5. Best Clinical-Strength Peptide Blend

1
BEST ALPHA-CASOZEPINE CALM

Zylkene Capsules for Dogs

Vetoquinol

Zylkene holds a best-in-class position for peptide-based calming because it delivers a purified milk-derived peptide (alpha-casozepine) with a non-sedating, mechanism-driven effect that many clinicians prefer for long-term anxiety management. Compared with the other amino-acid and botanical-focused options in this list, Zylkene’s distinct peptide technology offers a targeted pharmacologic approach that can reduce reliance on pharmaceuticals — a technical advantage that can translate to lower overall treatment cost for chronic cases despite a premium per-unit price.

4.4
★★★★☆
  • Milk-protein calm — moo

  • Non-drowsy ease

  • Milk-protein calm — moo

  • Non-drowsy ease

Review Summary

86%

"Users report Zylkene gently reduces mild anxiety and stress in many dogs with minimal side effects, though it can take several days to weeks and may not be strong enough for severe cases."

  • Openable capsules

  • Contains alpha-casozepine, a milk‑derived peptide shown to promote calm in dogs.

  • Openable capsules

  • Contains alpha-casozepine, a milk‑derived peptide shown to promote calm in dogs.

Increased Safety & Security

Zylkene holds a best-in-class position for peptide-based calming because it delivers a purified milk-derived peptide (alpha-casozepine) with a non-sedating, mechanism-driven effect that many clinicians prefer for long-term anxiety management. Compared with the other amino-acid and botanical-focused options in this list, Zylkene’s distinct peptide technology offers a targeted pharmacologic approach that can reduce reliance on pharmaceuticals — a technical advantage that can translate to lower overall treatment cost for chronic cases despite a premium per-unit price.

  • Milk-protein calm — moo

  • Non-drowsy ease

  • Openable capsules

  • Contains alpha-casozepine, a milk‑derived peptide shown to promote calm in dogs.

  • Available in capsules that can be given whole or opened and mixed with food for easy dosing.

  • Milk-protein calm — moo

  • Non-drowsy ease

  • Openable capsules

  • Contains alpha-casozepine, a milk‑derived peptide shown to promote calm in dogs.

  • Available in capsules that can be given whole or opened and mixed with food for easy dosing.

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From 35.96$
2
BEST VET-FORMULATED TRYPTOPHAN BLEND

Purina Pro Plan Veterinary Supplements Calming Care

Purina Pro Plan Veterinary Supplements Calming Care

Purina Pro Plan Veterinary Supplements Calming Care is a market-leading amino-acid focused option because it pairs clinically oriented formulation with Purina’s scale and veterinary distribution, making it widely available and often the most cost-effective choice for routine calming support. Versus boutique peptide or premium veterinary-only brands on this list, Purina’s financial advantage is predictable retail pricing and broad vet endorsement, while its technical strength lies in formulation and feeding-trial-backed development.

4.1
★★★★☆
  • Amino-acid based

  • Treat-like delight

  • Amino-acid based

  • Treat-like delight

Review Summary

80%

"Most buyers find Purina Pro Plan Veterinary Calming Care reasonably effective for situational anxiety and easy to dose, but some say it offers only modest benefit compared with stronger options."

  • Veterinary-formula

  • Veterinary-formulated supplement focused on behavior support using amino acids such as L-tryptophan.

  • Veterinary-formula

  • Veterinary-formulated supplement focused on behavior support using amino acids such as L-tryptophan.

Increased Safety & Security

Recreational Enjoyment

Time-Saving Convenience

Purina Pro Plan Veterinary Supplements Calming Care is a market-leading amino-acid focused option because it pairs clinically oriented formulation with Purina’s scale and veterinary distribution, making it widely available and often the most cost-effective choice for routine calming support. Versus boutique peptide or premium veterinary-only brands on this list, Purina’s financial advantage is predictable retail pricing and broad vet endorsement, while its technical strength lies in formulation and feeding-trial-backed development.

  • Amino-acid based

  • Treat-like delight

  • Veterinary-formula

  • Veterinary-formulated supplement focused on behavior support using amino acids such as L-tryptophan.

  • Tablet format dosed by weight for daily or sustained use under veterinary guidance.

  • Amino-acid based

  • Treat-like delight

  • Veterinary-formula

  • Veterinary-formulated supplement focused on behavior support using amino acids such as L-tryptophan.

  • Tablet format dosed by weight for daily or sustained use under veterinary guidance.

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$25-60 CAD

3
BEST L-THEANINE CHEWABLE

VetriScience Composure Pro Chews

VetriScience Composure Pro Chews

VetriScience Composure Pro Chews earns a top position for delivering concentrated amino-acid support in a palatable, chewable format designed for acute stress events and everyday anxiety; this product emphasizes convenient administration and high owner compliance. Compared with powder or capsule competitors here, its technical advantage is a flavor-forward chew and often higher per-dose potency—making it a premium but practical choice for dogs that refuse pills or require fast-acting oral delivery.

4.6
★★★★☆
  • Clinically-backed calm

  • Fast-acting chews

  • Clinically-backed calm

  • Fast-acting chews

Review Summary

88%

"Owners generally praise VetriScience Composure Pro Chews as palatable and often fast-acting for travel and noise-related anxiety, though a minority report limited effect on severe, chronic issues."

  • Lab-coat calm

  • Features clinically studied L-theanine (Suntheanine) to promote relaxation without sedation.

  • Lab-coat calm

  • Features clinically studied L-theanine (Suntheanine) to promote relaxation without sedation.

Increased Safety & Security

Time-Saving Convenience

VetriScience Composure Pro Chews earns a top position for delivering concentrated amino-acid support in a palatable, chewable format designed for acute stress events and everyday anxiety; this product emphasizes convenient administration and high owner compliance. Compared with powder or capsule competitors here, its technical advantage is a flavor-forward chew and often higher per-dose potency—making it a premium but practical choice for dogs that refuse pills or require fast-acting oral delivery.

  • Clinically-backed calm

  • Fast-acting chews

  • Lab-coat calm

  • Features clinically studied L-theanine (Suntheanine) to promote relaxation without sedation.

  • Palatable soft chews that are easy to give daily or during acute stress events like fireworks or travel.

  • Clinically-backed calm

  • Fast-acting chews

  • Lab-coat calm

  • Features clinically studied L-theanine (Suntheanine) to promote relaxation without sedation.

  • Palatable soft chews that are easy to give daily or during acute stress events like fireworks or travel.

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$45-85 CAD

4
BEST BUDGET CALMING CHEW

NaturVet Quiet Moments Calming Aid

NaturVet Quiet Moments Calming Aid

NaturVet Quiet Moments is positioned as the value leader among amino-acid and calming supplements by combining basic amino acids and calming botanicals into an accessible, budget-friendly formula for owners exploring non-prescription options. While it generally lacks the clinical depth of peptide-focused or veterinary-grade alternatives on this list, its financial advantage is clear: lower cost of entry and widespread retail availability, which makes it a sensible first-line trial for mild situational anxiety.

3.9
★★★☆☆
  • Herbal-amino blend

  • Nighttime gentle calm

  • Herbal-amino blend

  • Nighttime gentle calm

Review Summary

74%

"Quiet Moments is regarded as an affordable calming aid that helps some dogs relax, but reviews are mixed with many users reporting little to no change for more serious anxiety."

  • Budget-friendly zzz

  • Economical chewable option designed for situational calming and short-term stress relief.

  • Budget-friendly zzz

  • Economical chewable option designed for situational calming and short-term stress relief.

Increased Safety & Security

Recreational Enjoyment

NaturVet Quiet Moments is positioned as the value leader among amino-acid and calming supplements by combining basic amino acids and calming botanicals into an accessible, budget-friendly formula for owners exploring non-prescription options. While it generally lacks the clinical depth of peptide-focused or veterinary-grade alternatives on this list, its financial advantage is clear: lower cost of entry and widespread retail availability, which makes it a sensible first-line trial for mild situational anxiety.

  • Herbal-amino blend

  • Nighttime gentle calm

  • Budget-friendly zzz

  • Economical chewable option designed for situational calming and short-term stress relief.

  • Formulated for easy administration and broad availability in pet stores and online.

  • Herbal-amino blend

  • Nighttime gentle calm

  • Budget-friendly zzz

  • Economical chewable option designed for situational calming and short-term stress relief.

  • Formulated for easy administration and broad availability in pet stores and online.

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$12-30 CAD

5
BEST CLINICAL-STRENGTH PEPTIDE BLEND

Nutramax Solliquin Behavioral Health Supplement

Nutramax Solliquin Behavioral Health Supplement

Nutramax Solliquin is regarded as a veterinary-grade market leader for behavioral health because it integrates amino-acid support with a proprietary complementary blend aimed at mood and coping behaviors, backed by Nutramax’s veterinary distribution and product support. Compared with simpler OTC or single-ingredient options in this list, Solliquin’s technical advantage is its vet-focused dosing and formulation rigor; financially, it occupies a premium segment but can reduce the need for prescription drugs in some patients, offering long-term value for complex cases.

4.4
★★★★☆
  • Dual amino blend

  • Behavioral support long-term

  • Dual amino blend

  • Behavioral support long-term

Review Summary

85%

"Solliquin is frequently recommended by veterinarians and reviewers for reliably reducing separation and noise-related anxiety, though it’s more expensive and can take a week or more to show full effect."

  • Science-meets-zen

  • Veterinary-grade behavioral supplement combining targeted amino acids and peptide support for mood regulation.

  • Science-meets-zen

  • Veterinary-grade behavioral supplement combining targeted amino acids and peptide support for mood regulation.

Increased Safety & Security

Self-Improvement & Personal Growth

Nutramax Solliquin is regarded as a veterinary-grade market leader for behavioral health because it integrates amino-acid support with a proprietary complementary blend aimed at mood and coping behaviors, backed by Nutramax’s veterinary distribution and product support. Compared with simpler OTC or single-ingredient options in this list, Solliquin’s technical advantage is its vet-focused dosing and formulation rigor; financially, it occupies a premium segment but can reduce the need for prescription drugs in some patients, offering long-term value for complex cases.

  • Dual amino blend

  • Behavioral support long-term

  • Science-meets-zen

  • Veterinary-grade behavioral supplement combining targeted amino acids and peptide support for mood regulation.

  • Often recommended for chronic anxiety and behavioral issues and used as part of a multi-modal treatment plan.

  • Dual amino blend

  • Behavioral support long-term

  • Science-meets-zen

  • Veterinary-grade behavioral supplement combining targeted amino acids and peptide support for mood regulation.

  • Often recommended for chronic anxiety and behavioral issues and used as part of a multi-modal treatment plan.

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$55-120 CAD

What the Research Says About Amino Acids and Peptides for Canine Calm

A growing body of veterinary research examines how specific amino acids and bioactive peptides can influence brain chemistry and behavior. While responses vary by dog and situation, several agents in this category have plausible mechanisms or clinical data supporting calming effects. Important caveats include variable quality of studies, differences in formulations and doses, and the fact that these supplements are most effective when used alongside behavior modification, environmental management, and veterinary guidance.

Alpha-casozepine (a milk-derived peptide used in products like Zylkene) has been evaluated in clinical trials and case reports showing reductions in situational stress behaviors in some dogs, supporting its use as a targeted peptide option.

L-theanine is an amino acid that modulates neurotransmitter activity and autonomic responses; controlled studies and veterinarian reports suggest it can promote relaxation without sedation when used at appropriate doses.

L-tryptophan, a serotonin precursor, can influence mood and reactivity; research indicates benefit in certain behavior contexts, especially when dietary intake is managed and dosing follows veterinary recommendations.

GABA supplementation aims to increase inhibitory signaling, but oral GABA has mixed evidence because of limited blood-brain barrier crossing; some formulations combine GABA with other cofactors to enhance practical effects.

Clinical evidence is strongest when supplements are part of a multimodal plan: supplements, behavior modification, enrichment, and, if needed, prescription medications under veterinary supervision.

Study quality and regulatory labeling vary between products and regions; look for third-party manufacturing standards (GMP), published research references, and veterinarian support when selecting a supplement in Canada.

Across Canada in 2025, amino acid and peptide calming supplements offer practical, science-informed options for owners looking to reduce stress-related behaviors. The five products featured on this page — Zylkene Capsules for Dogs, Purina Pro Plan Veterinary Supplements Calming Care, VetriScience Composure Pro Chews, NaturVet Quiet Moments Calming Aid, and Nutramax Solliquin Behavioral Health Supplement — represent a range of peptide- and amino-acid-centered approaches and dosing formats. For targeted peptide support with a notable clinical history, Zylkene Capsules for Dogs are often the best choice for owners prioritizing alpha-casozepine-based support; however, each dog's needs differ and other options like Purina Pro Plan Veterinary Supplements Calming Care or VetriScience Composure Pro Chews may suit specific situations better. We hope you found the information you were looking for — refine or expand your search by brand, active ingredient, or symptom using the site search to narrow to the best match for your dog.

Discover More
  • Herbal Calming Supplements for Dogs
  • Calming Chews and Functional Treats for Dogs
  • Calming Tablets and Capsules for Dogs
  • Calming Powders and Liquid Supplements for Dogs
  • Targeted Anxiety Support for Separation and Noise Phobias in Dogs

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